• Scientific research level
According to the needs of customers, customized R&D can be carried out to produce scientific research grade retrovirus vectors within 500mL. It provides the whole solution of CDMO service from antibody screening, vector construction, viral vector packaging to CAR-T cell preparation and CAR-T cell activity verification in vivo and in vitro. At the same time, the company can provide CAR specific retroviral vector products targeting a variety of tumor targets, such as BCMA, CD19, CD38, CD22, EGFR/EGFRV III, which can efficiently transduce human primary T cells. Among them, CD38 CAR is the whole human anti-CD38 scFv structure screened by our phage display scFv antibody library, with independent intellectual property rights.

     • Clinical level
Mass production of GMP grade retroviral vectors that meet the application regulatory standards is used to prepare CAR-T, CAR-NK or CAR-M cells, which is crucial for IIT (clinical trial initiated by PI), IND application, clinical Phase I-III clinical trial and large-scale commercial production of immunocellular therapy. Cell Valley can provide services such as production, purification and batch inspection of retroviral vectors that meet GMP specifications. Our GMP grade retroviral vector production service adopts the production process with Junhou Biological's independent intellectual property rights, including the production of stable retroviral vector production cell bank working cell bank (WCB) and clinical grade retroviral vector production. The GMP grade retroviral vector production service of Cell Valley ensures that the products meet GMP standards, and the viral vector titer is>2 × 107 TU/mL, quality control meets IIT test and IND declaration

     • New SE retroviral vector
The new SE retrovirus vector contains double promoters, which can co express foreign genes with shRNA or microRNA, and the packaging capacity is>6kb. According to the needs of customers, Cell Valley can customize the R&D and production of new SE scientific research grade retroviral vectors with a single batch of less than 500mL, and provide the overall solution of CDMO services in the whole process from antibody screening, shRNA design of different genes, new vector construction, viral vector packaging, CAR-T cell preparation, and CAR-T cell in vivo and in vitro viability verification. At the same time, it can provide specific SE retrovirus vector products co expressed with CAR gene and LSD1 shRNA, HPK1 shRNA, TOX shRNA, PTPN2 shRNA or microRNA targeting a variety of tumor targets, such as BCMA, CD19, CD38, etc.

Retrovirus, also known as retrovirus, is an RNA virus with long terminal repeat (LTR) at its two ends. The virus is spherical, enveloped, and has spikes on its surface. The particle diameter is about 100 nm. The retrovirus genome has three genes: Gag encodes the core protein of the virus; Pol - encodes reverse transcriptase; Env encodes the envelope glycoprotein of the virus.